Abstract: Aiming at the different requirements of data transmission in wireless sensor networks, QoS was divided into three services. By using game theory, the relationship among the delay, reliability and energy consumption during the process of data transmission was analyzed. Based on ACS (ant colony system), ACS based differentiated service and game-theory routing (ADSGR) was proposed. In accordance with the diverse QoS requirements, the ADSGR chooses the suitable paths, and improves network performance and resource utility. Experimental results show the ADSGR has better performance than some other routing algorithms in terms of the delay, reliability and energy consumption.